Description

: For non-deletional α-thalassemia, Sanger DNA sequencing can be used to detect all point mutations. Allele-specific PCR also has a role in areas where certain mutations are common, such as hemoglobin Constant Spring in Southeast Asia. DNA analysis of the common α-globin gene deletions performed by multiplex polymerase chain reaction (PCR) followed by agarose gel electrophoresis may, on occasion, be used for confirmatory purposes.

Polymerase chain reaction (PCR)

5 business days

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